Construction of a Barley Gene Regulatory Network for Phenolic Metabolism Using Mutants and Near-Isogenic Lines

Olesya Shoeva¹, Ksenia Strygina², Anastasiia Glagoleva³, Tatjana Kukoeva⁴, Elena Gordeeva⁵, Shakhira Zakhrabekova⁶, Mats Hansson⁷, Elena Khlestkina^8
1ICG SB RAS, Novosibirsk, Russia, olesya_ter@bionet.nsc.ru
²VIR, St. Petersburg, Russia, k.strygina@vir.nw.ru
³ICG SB RAS, Novosibirsk, Russia, glagoleva@bionet.nsc.ru
⁴ICG SB RAS, Novosibirsk, Russia, kukoeva@bionet.nsc.ru
⁵ICG SB RAS, Novosibirsk, Russia, elgordeeva@bionet.nsc.ru
⁶Lund University, Lund, Sweden, shakhira.zakhrabekova@biol.lu.se
⁷Lund University, Lund, Sweden, mats.hansson@biol.lu.se
⁸ICG SB RAS, Novosibirsk, Russia; VIR, St. Petersburg, Russia, khlest@bionet.nsc.ru

In the present study, the components of the gene regulatory network governing the anthocyanin and proanthocyanidin biosynthesis in barely grain and leaf sheath were identified based on comparative mapping and transcription analysis approaches. The regulatory relationships between the genes were established using unique barley anthocyanin/proanthocyanidin mutants and near-isogenic lines.