Evolution and diversity of uORFs in mammals

Alla Fedorova¹, Pasha Baranov^2
1University College Cork, School of Biochemistry and Cell Biology, fedorovaad1995@yandex.ru
²University College Cork, School of Biochemistry and Cell Biology, P.Baranov@ucc.ie

5’ regions which are located upstream of CDS (coding sequence) in transcripts have been thought to be untranslated for a long time and therefore have been referred as 5’UTRs (untranslated regions). However, some of them may contain translated parts which are known as uORFs (upstream open reading frames). Some uORFs can participate in regulatory mechanisms of downstream translation and there are known mechanisms of such regulation. However, there are still plenty of uORFs with unknown function or still undiscovered and mis-annotated. In this work we investigated diversity and evolution of uORFs at a genome-wide level using the advent of Ribo-seq method (robosome profiling) and different databases and software.