Influence of the Immobilized Subtilisins on Perfomance Indicators of the Rat Heart in Experiment

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Pavel Madonov¹, Roman Knyazev², Konstantin Ershov^3
1Department of Experimental Pharamology Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russian Federation Department of Pharmacology, Clinical Pharmacology and Evidence-Based Medicine Novosibirsk State Medical University Novosibirsk, Russia Federation, Pmadonov@yandex.ru
²Research Institute of Biochemistry – a branch of the Federal Research Center for Fundamental and Translational Medicine Department of Pharmacology, Clinical Pharmacology and Evidence-Based Medicine Novosibirsk State Medical University Novosibirsk, Russian Federation, Knjazev_roman@mail.ru
³Department of Pharmacology, Clinical Pharmacology and Evidence-Based Medicine Novosibirsk State Medical University, Novosibirsk, Russian Federation, Ershov_k@bk.ru

Abstract The immobilized subtilisins are bacterial proteinases with a high thrombolytic activity, capable of improving peripheral blood circulation by dissolving the blood clot and reducing manifestations of endothelial dysfunction. This article presents the materials of the effect of immobilized subtilisins on performance heart indicators in the experiment. В The action of these proteinases was studied on a model of isolated rat heart according to Langendorff. Indicators of heart rate (HR), pressure developed by the left ventricle (LVP), heart efficiency (HE) were selected for registration. It was shown when adding the immobilized subtilisins, an increase of the left ventricular contraction force and a decrease in a heart rate were observed. The results indicate that immobilized subtilisins have positive inotropic and negative chronotropic effects.