Opisthorchis felineus extracellular vesicles increase cell proliferation and migration rates of human H69 cholangiocytes

Poster (download)

317

D.V. Ponomarev¹, O. Zaparina², M.Y. Pakharukova³, V.A. Mordvinov^4
1Laboratory of Molecular Mechanisms of Pathological Processes Institute of Cytology and Genetics SB RAS, p.dmitr@outlook.com
²Laboratory of Molecular Mechanisms of Pathological Processes Institute of Cytology and Genetics SB RAS, zp.oksana.93@gmail.com
³Laboratory of Molecular Mechanisms of Pathological Processes Institute of Cytology and Genetics SB RAS, pmaria@yandex.ru
⁴Laboratory of Molecular Mechanisms of Pathological Processes Institute of Cytology and Genetics SB RAS, mordvin@bionet.nsc.ru

Opisthorchis felineus, is a food-borne liver trematode and the main cause of opisthorchiasis in Russia and Europe. It affects hepatobiliary system of fish-eating mammals, including humans. Opisthorchiasis is associated with chronic inflammation, biliary epithelium proliferation, liver fibrosis, and even might cause cholangiocarcinoma among chronically infected individuals. Secreted proteins and extracellular vesicles of liver flukes might play an important role in the development of pathology. We investigated the response of human H69 cholangiocytes and human hepatoma HepG2 cells to adult liver flukes and to extracellular vesicles released from the flukes. We have demonstrated high mitogenic and cell migration stimulating activity of flukes and EVs vesicles. The activity was specific for cholangiocytes, but not for HepG2 cells. Specific mitogenic effect of liver fluke extracellular vesicles on proliferation and migration of human cholangiocytes in vitro may reflect the mechanisms of development of precancerous biliary intraepithelial neoplasia during opisthorchiasis in vivo.