Possible effect of SNP TATA-boxes of human erythropoiesis gene promoters on cognitive disorders

Poster (download)

444

Ekaterina Sharypova¹, Irina Drachkova², Irina Chadaeva³, Mikhail Ponomarenko⁴, Ludmila Savinkova^5
1Institute of Cytology and Genetics of SB RAS, sharypova@bionet.nsc.ru
²Institute of Cytology and Genetics of SB RAS, drachkova@bionet.nsc.ru
³Institute of Cytology and Genetics of SB RAS, ichadaeva@bionet.nsc.ru
⁴Institute of Cytology and Genetics of SB RAS, pon@bionen.nsc.ru
⁵Institute of Cytology and Genetics of SB RAS, lksav@bionet.nsc.ru

In recent years, there is increasing evidence that various forms of anemia, changes in the quantity and quality of blood cells, may be involved in the pathogenesis of various cognitive disorders accompanying Alzheimer’s and Parkinson’s diseases, depression of various degrees, etc. In addition to erythroid cells, hemoglobin has been shown to be widely expressed in non-erythroid cells, including neurons of different parts of the brain.В  We analyzed in silico and in vitro unannotated SNPs in TATA boxes of human genes involved in erythropoiesis. Experimental verification in vitro using the method of electrophoretic mobility shift assay (EMSA) showed the correspondence of prognosis and experimental data. The obtained estimates of the effect of TATA box SNP markers on the formation of TBP/TATA complexes make it possible to consider some SNP markers of erythroid genes as markers of cognitive disorders.