Promoter expression landscape in skeletal muscle in hindlimb suspension and recovery model in rat

Poster (download)

342

Guzel Gazizova¹, Ruslan Deviatiiarov², Islam Nigmetzyanov³, Oksana Tyapkina⁴, Leniz Nurullin⁵, Oleg Gusev^6
1Kazan Federal University, grgazizova@gmail.com
²Kazan Federal University, ruselusalbus@gmail.com
³Kazan Federal University, islamka32@gmail.com
⁴Federal Research Center “Kazan Scientific Center of the RAS\”, antitoxin@icloud.com
⁵Federal Research Center “Kazan Scientific Center of the RAS\”, leniz2009@gmail.com
⁶Kazan Federal University, RIKEN, oleg.gusev@riken.jp

Loss of muscle mass and function during long period of physical inactivity still remains a clinical problem for humanity because the significant reducing of life quality and increasing mortality. In recent studies global gene expression in atrophied and recovered skeletal muscles on different animal models was analyzed. However, previously whole-genome regulation of atrophy and following recovery at promoter and enhancer level has not been studied. To identify transcription initiation sites (TSS), and evaluate full-genome RNA expression at the level of individual promoters and enhancers during unloading and subsequent recovery in rats two types of muscles, \”slow\” (m. Soleus) and \”fast\” (m. EDL), were examined in rats in normal conditions, after 1, 3 and 7 days of hindlimb suspension and following 1, 3 and 7 days of recovery using CAGE (Cap Analysis of Gene Expression) method followed by Illumina HiSeq 2500 sequencing. We obtained 9971 unique CAGE clusters, among which 6377 were promoters associated with genes. Differential expression of genes and their promoter activity were strongly varied in m. EDL and m. soleus within suspension-recovery time course. This study provides the first systematic annotation of promoters landscape and genes activated in \”fast\” and \”slow\” muscle types under induced atrophy and following recovery in rats.