Cluster organization of miRNA binding sites in mRNA of atherosclerosis candidate genes

Mukushkina Dina1, Aisina Dana2, Ivashchenko Anatoliy31SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, dina.mukushkina@gmail.com2SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, dana.aisina03@gmail.com3SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, a.iavashchenko@gmail.com Many candidate genes are involved in the development of atherosclerosis, which are manifested in a variety of metabolic processes, disturbances of which are observed in this disease. Many publications describe the involvement of miRNAs in the development of atherosclerosis. Therefore, it is necessary to establish which miRNAs can affect the expression of candidate genes. Quantitative characteristics of the interaction of miRNAs with mRNAs candidate genes were determined using the MirTarget program, which detects localization of miRNA binding sites in 3\’UTR, 5\’UTR and CDS; free energy interaction miRNA with mRNA; schemes of nucleotide interaction between miRNA and mRNA. In the mRNA of atherosclerosis genes, the binding sites of two or more miRNAs located in 3\’UTR, 5\’UTR and CDS are established. The organization of miRNAs binding sites with overlapping nucleotide sequences forming clusters was revealed. Such an organization of miRNAs binding sites leads to their compaction several times and causes competition among miRNAs for binding to mRNA. The features of the interaction of miRNAs with mRNA of candidate genes depending on their expression are established. Associations of miRNAs and candidate atherosclerosis genes are proposed for the early diagnosis of this disease.

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The prevalence and risk factors of carotid artery stenosis in type 2 diabetic patients.

Poster (download) Elena Koroleva1, Rustam Khapaev2, Alexandr Lykov3, Vadim Klimontov41Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, ekoro@bk.ru2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, khapaevrs@bionet.nsc.ru3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, aplykov2@mail.ru4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, klimontov@mail.ru Abstract Background and aim: Diabetic patients with carotid artery stenosis have an exceptional high risk for all-cause and cardiovascular death. This study aimed to determine the prevalence and risk factors of carotid stenosis in patients with type 2 diabetes (T2D). Materials and Methods: 390 patients with T2D were examined. Duplex ultrasound of the extracranial carotid arteries was performed and the intima-media thickness of the common carotid arteries (IMT) was determined. A study of serum concentrations of NO, calponin-1, relaxin, and L-citrulline was performed in 152 patients. Results: the signs of carotid arteriosclerosis were revealed in 338 patients (86%). 134 patients (33%) had carotid stenosis. The patients with carotid artery stenosis (group 2, n = 134), as compared to those without (group 1, n = 204) were older, had a larger waist circumference, a longer diabetes duration, higher urinary albumin/creatinine ratio, and lower eGFR, all p<0.05. There were no differences in sex distribution, smoking rate and other laboratory parameters between the groups. In group 2 NO and citrulline concentrations were significantly higher than in group 1 (p=0.005 and p=0.003 respectively). According to ROC analysis, the age ≥65 years, T2D duration ≥15 years and high serum NO concentration were determined as the risk factors for carotid stenosis. The decrease in eGFR <60 ml/min*1.73 m2, diabetic retinopathy, myocardial infarction in medical history increased the risk of stenosis (HR 2.24; p=0.0006, HR 1.88; p=0.006, and HR 2.64; p=0.002, respectively). Conclusions: the study shows a high prevalence of carotid artery stenosis in patients with T2D. The age ≥65 years, T2D duration ≥15 years, GFR <60 ml/min*1.73 m2, diabetic retinopathy and myocardial infarction are risk factors for the carotid artery stenosis in patients with T2D.   Введите абстрактную аннотацию ТОЛЬКО здесь

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