Poster (download) Elena Pushkova1, George Krasnov2, Roman Novakovskiy3, Liubov Povkhova4, Artemy Beniaminov5, Nadezhda Bolsheva6, Tatiana Rozhmina7, Alexey Dmitriev8, Nataliya Melnikova91Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, pushkova18@gmail.com2Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, gskrasnov@mail.ru3Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, 0legovich46@mail.ru4Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Russia, povhova.lv@phystech.edu5Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, abeniaminov@mail.ru6Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, nlbolsheva@mail.ru7Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; Federal Research Center for Bast Fiber Crops, Torzhok, Russia, tatyana_rozhmina@mail.ru8Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, alex_245@mail.ru9Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, mnv-4529264@yandex.ru Flax (Linum usitatissimum L.) is an agriculturally important plant that has a wide range of applications in industry, and the direction of cultivar application is determined by its genetic characteristics. The present work aimed to obtain high-quality sequences of flax genomes and transcriptomes for genetically diverse cultivars and lines, which have breeding value and different direction of use. Six cultivars/lines (LM98, #3896, Diplomat, Atlant, Universal, Alizee) were selected for the present work. We have developed an optimal method for the extraction of pure high-molecular-weight DNA from flax plants and performed genome and transcriptome sequencing. From 6 to 10 Gb was obtained for each of the studied flax cultivars/lines on the Oxford Nanopore platform and 20-25 million reads on the Illumina platform. Transcriptome sequencing of different flax tissues was also performed. Genome assembly using Flye resulted in the N50 value from 200 kb to 1 Mb depending on the genotype. Genome annotation was then performed. The obtained genome assemblies are the basis for molecular genetic studies in flax and allow assessment of the differences in L. usitatissimum cultivars/lines at the genome-wide level.