Zulfiia Guvatova1, George Krasnov2, Anastasiya Snezhkina3, Artemy Tokarev4, Maria Fedorova5, Anna Kudryavtseva61Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, guvatova.zulfiya@mail.ru2Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, gskrasnov@mail.ru3Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, leftger@rambler.ru4Artemy Tokarev Veterinary biology faculty MSAVM&B – MVA named after K.I. Skryabin Moscow, Russia, artemiy.tokarev@mail.ru5Laboratory of Postgenomic Research Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, fedorowams@yandex.ru6Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, rhizamoeba@mail.ru Nothobranchius rachovii is a species of freshwater annual killifish native to Africa. This organism is an attractive model in aging studies because of short lifetime and rapid maturation. In this study, we present draft genome assembly of this organism based on Nanopore reads and polished with Illumina reads. We tested four popular Nanopore reads assemblers (Flye, MaSuRCA, Shasta, wtdbg2) and two polishers (Pilon and MaSuRCA-polish tool). We selected the best combination of these tools in terms of contig length and assembly completeness.
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