Genome Assembly and Annotation of Nothobranchius rachovii killifish

Zulfiia Guvatova1, George Krasnov2, Anastasiya Snezhkina3, Artemy Tokarev4, Maria Fedorova5, Anna Kudryavtseva6
1Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, guvatova.zulfiya@mail.ru
2Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, gskrasnov@mail.ru
3Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, leftger@rambler.ru
4Artemy Tokarev Veterinary biology faculty MSAVM&B – MVA named after K.I. Skryabin Moscow, Russia, artemiy.tokarev@mail.ru
5Laboratory of Postgenomic Research Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, fedorowams@yandex.ru
6Center for Precision Genome Editing and Genetic Technologies for Biomedicine Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow, Russia, rhizamoeba@mail.ru

Nothobranchius rachovii is a species of freshwater annual killifish native to Africa. This organism is an attractive model in aging studies because of short lifetime and rapid maturation. In this study, we present draft genome assembly of this organism based on Nanopore reads and polished with Illumina reads. We tested four popular Nanopore reads assemblers (Flye, MaSuRCA, Shasta, wtdbg2) and two polishers (Pilon and MaSuRCA-polish tool). We selected the best combination of these tools in terms of contig length and assembly completeness.

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