Semi-quantitative analysis of serum proteome in patients with bipolar disorder

Poster (download) Seregin Alexander Alexandrovich1, Smirnova Lyudmila Pavlovna2, Simutkin German Gennadievich31Mental Health Research Institute Tomsk National Research Medical Center of the Russian Academy of Sciences Tomsk, Russia, apocalips1991@mail.ru2Mental Health Research Institute Tomsk National Research Medical Center of the Russian Academy of Sciences Tomsk, Russia, lpsmirnova@yandex.ru3Mental Health Research Institute Tomsk National Research Medical Center of the Russian Academy of Sciences Tomsk, Russia, ggsimutkin@gmail.com The work analyzed the protein spectrum of blood serum of 45 with bipolar disorder. Protein recruitment in BD was mainly associated with the immune response, regulation of transport processes through the cell membrane and cellular communication, the development of neurons and oligodendrocytes, and cell growth. Cadherin-5 and vascular endothelial growth factor receptor 1 are a marker of endothelial dysfunction. The detection of these proteins suggests the presence of endothelial dysfunction in the pathogenesis of BD. The detection of myelin 1 transcription factor and the literature data on the effect of mutations in the MYT1L gene on the risk of major depressive disorder suggest the MYT1 protein as a potential BD biomarker. The results may help to discover new pathways associated BD.

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Genomic analyses of Novymonas esmeraldas and Ca. Pandoraea novymonadis

Poster (download) Alexandra Zakharova1, Daria Tashyreva2, Jorge Morales3, Eva Nowack4, Julius LukeЕЎ5, Vyacheslav Yurchenko61Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic, alexandraz.6946@gmail.com2Institute of Parasitology, Biological Centre, ДЊeskГ© BudД›jovice, Czech Republic, tashyreva@paru.cas.cz3Heinrich Heine University, DГјsseldorf, Germany, jjfreites@hotmail.com4Heinrich Heine University, DГјsseldorf, Germany, E.Nowack@uni-duesseldorf.de5Institute of Parasitology, Biological Centre, ДЊeskГ© BudД›jovice, Czech Republic, jula@paru.cas.cz6Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic, Vyacheslav.Yurchenko@osu.cz Members of the family Trypanosomatidae are intensively studied because some of them are infectious agents of dangerous human ailments, such as Chagas disease, African sleeping sickness and leishmaniasis. It has been recently demonstrated that representatives of 4 trypanosomatid genera of 2 subfamilies [Leishmaniinae (genus Novymonas) and Strigomonadinae (genera Angomonas, Strigomonas and Kentomonas)] harbor bacterial symbionts. The endosymbiont of Novymonas esmeraldas is Ca. Pandoraea novymonadis. This symbiotic system is of particular interest, since none of the participants has close relatives involved in such relationships, indicating its independent and relatively recent origin. In this work, we have investigated genomes and proteomes of N. esmeraldas and Ca. Pandoraea novymonadis. We found and investigated factors involved in the symbiotic interactions. Biochemical analysis demonstrated that numerous host-encoded proteins were enriched in the bacterial fraction, represented by ОІ-oxidation enzymes, transporters, transmembrane proteins and others. We have found that members of this endosymbiotic system may be a good model to study the establishment and development of symbiosis between bacteria and protists.

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Quantitative differences in the proteomic composition of the blood serum of patients with simple and paranoid schizophrenia

Poster (download) Video (download) Smirnova Liudmila, Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center, Tomsk, Russia, lpsmirnova@yandex.ru Semke Arkadiy, the Department of Endogenous Disorders Mental Health Research Institute of Tomsk National Research Medical Center. Tomsk, Russia, arkan1959@mail.ru Zgoda Victor, Laboratory of systems biology, Institute of Biomedical Chemistry, Moscow, Russia, vic@ibmh.msk.su Ivanova Svetlsna, Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center, Tomsk, Russia, ivanovaniipz@gmail.com Dmitrieva Elena, Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center, Tomsk, Russia, lena-312tom@yandex.ru The work analyzed the protein spectrum of blood serum of simple and paranoid schizophrenia. A set of proteins in schizophrenia was mainly associated with responsible for protein synthesis and the processes of transduction and translation; immune response, oxidative stress and apotosis, cell compataminization and cell contacts. In the blood serum of patients in the general group of schizophrenia, a significant increase in the number of RIPK1, mGluR6 receptors was revealed in comparison with healthy people. In addition, the amount of mGluR6 was significantly higher in patients with a simple form of schizophrenia than in patients with a paranoid form (p = 0.021). A quantitative assessment of the specific minor proteins that we studied using labeled standard peptides showed an increase in their number depending on the severity of the disease (a simple form of schizophrenia, leading negative symptoms)

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