Genomic analyses of Novymonas esmeraldas and Ca. Pandoraea novymonadis

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Alexandra Zakharova¹, Daria Tashyreva², Jorge Morales³, Eva Nowack⁴, Julius LukeЕЎ⁵, Vyacheslav Yurchenko⁶
¹Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic, alexandraz.6946@gmail.com
²Institute of Parasitology, Biological Centre, ДЊeskГ© BudД›jovice, Czech Republic, tashyreva@paru.cas.cz
³Heinrich Heine University, DГјsseldorf, Germany, jjfreites@hotmail.com
⁴Heinrich Heine University, DГјsseldorf, Germany, E.Nowack@uni-duesseldorf.de
⁵Institute of Parasitology, Biological Centre, ДЊeskГ© BudД›jovice, Czech Republic, jula@paru.cas.cz
⁶Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic, Vyacheslav.Yurchenko@osu.cz

Members of the family Trypanosomatidae are intensively studied because some of them are infectious agents of dangerous human ailments, such as Chagas disease, African sleeping sickness and leishmaniasis. It has been recently demonstrated that representatives of 4 trypanosomatid genera of 2 subfamilies [Leishmaniinae (genus Novymonas) and Strigomonadinae (genera Angomonas, Strigomonas and Kentomonas)] harbor bacterial symbionts. The endosymbiont of Novymonas esmeraldas is Ca. Pandoraea novymonadis. This symbiotic system is of particular interest, since none of the participants has close relatives involved in such relationships, indicating its independent and relatively recent origin.

In this work, we have investigated genomes and proteomes of N. esmeraldas and Ca. Pandoraea novymonadis. We found and investigated factors involved in the symbiotic interactions. Biochemical analysis demonstrated that numerous host-encoded proteins were enriched in the bacterial fraction, represented by ОІ-oxidation enzymes, transporters, transmembrane proteins and others. We have found that members of this endosymbiotic system may be a good model to study the establishment and development of symbiosis between bacteria and protists.