Metabolic response of the Siberian frog Rana amurensis to anoxia

Sergei V. Shekhovtsov1, Nina A. Bulakhova2, Yuri P. Tsentalovich3, Ekaterina A. Zelentsova4, Daniil I. Berman51ICG SB RAS, Novosibirsk, Russia, shekhovtsov@bionet.nsc.ru2IBPN FEB RAS, Magadan, Russia, sigma44@mail.ru3ITC SB RAS, Novosibirsk, Russia, yura.tsentalovich@tomo.nsc.ru4ITC SB RAS, Novosibirsk, Russia, zelentsova.ekaterina@gmail.com5IBPN FEB RAS, Magadan, Russia, dberman@mail.ru The Siberian frog Rana amurensis is the only amphibian capable of surviving several months at almost complete anoxia. We performed 1H NMR metabolomics analysis for the liver and heart of the Siberian frog during normoxia and anoxia. We found that anoxia causes significant energetic stress with much less energy molecules available in the studied organs. This is accompanied by the accumulation of several end products, most significantly lactate, alanine, and succinate, as well as most amino acids.

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