Interplay between 5-HT and BDNF system in recombinant mouse strain upon chronic fluoxetine administration

Poster (download) Rodnyy A.Ya.1, Kondaurova E.M2, Antonov Y.V.3, Ilchibaeva T.V.4, Tsybko A.S.5, Naumenko V.S.61Institute of Cytology and Genetics Novosibirsk, Russia, aleksandr1994rodny@gmail.com2Institute of Cytology and Genetics Novosibirsk, Russia, kond_em@bionet.nsc.ru3Institute of Cytology and Genetics Novosibirsk, Russia, yegor@bionet.nsc.ru4Institute of Cytology and Genetics Novosibirsk, Russia, rbicehok@mail.ru5Institute of Cytology and Genetics Novosibirsk, Russia, antontsybko@bionet.nsc.ru6Institute of Cytology and Genetics Novosibirsk, Russia, naumenko2002@bionet.nsc.ru BDNF plays a key role in the development, differentiation, synaptogenesis and survival of brain neurons and in the processes of their adaptation to external impacts. Serotonergic (5-HT) system is another basic player in brain development and neuroplasticity. The study presents a comparative analysis of chronic fluoxetine treatment in recombinant mice differing in distal chromosome 13 fragment containing Htr1A gene of CBA mice strain on C57Bl6 genetic background. The problem here to be studied is mechanism of BDNF and 5-HT systems` interactions in antidepressant insensitivity. We measured mRNA and protein levels of BDNF, p75NTR, TrkB, 5-HT1A and 5-HT7 receptors, levels of 5-HT and its primary metabolite 5-Hydroxyindoleacetic acid (5-HIAA) in the brain structures that could have play primary role in mechanism of depression – frontal cortex and hippocampus. At the heart of the discussion are the different changes in BDNF system as well as in 5-HT system which allows us to conclude that the chronic fluoxetine injection increased depressive-like behavior in recombinant mice carrying distal chromosome 13 fragment containing Htr1A gene of CBA mice.

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Study of serotonin transporter gene polymorphism Stin2 in two Siberian indigenous populations

Poster (download) Mira Khantemirova1, Daria Lichman2, Daria Bazovkina3, Anatoly Bragin4, Vladimir Naumenko5, Ludmila Osipova61Laboratory of populational ethnogenetics, Institute of cytology and genetics; Laboratory of computer genomics, Novosibirsk State University Novosibirsk, Russia, hantemiramira@mail.ru2Laboratory of populational ethnogenetics, Institute of cytology and genetics; Laboratory of computer genomics, Novosibirsk State University Novosibirsk, Russia, daria.lichman@gmail.com3Laboratory of behavioral neurogenomics, Institute of cytology and genetics Novosibirsk, Russia, daryabazovkina@gmail.com4Laboratory of computer genomics, Novosibirsk State University Novosibirsk, Russia, ibragim@bionet.nsc.ru5Laboratory of behavioral neurogenomics, Institute of cytology and genetics Novosibirsk, Russia, naumenko2002@bionet.nsc.ru6Laboratory of populational ethnogenetics, Institute of cytology and genetics; Laboratory of computer genomics, Novosibirsk State University Novosibirsk, Russia, ludos77@yandex.ru Serotonin (5-HT) participates in regulation of emotions, mood, sleep, appetite, and cognitive processes. Serotonin transporter (5-HTT, SERT) modulates serotoninergic neurotransmission through reuptake of 5-HT from the synaptic cleft. A variable number tandem repeat polymorphism found in intron 2 of the 5-HTT gene (Stin2 polymorphism) influences 5-HTT expression and is associated with anxiety, depression, suicidal behavior, obsessive-compulsive disorder. The aim of the present study was to investigate Stin2 allele frequencies in populations of Tundra Nenets and Nganasans inhabiting north and east Siberia. The frequency of the STin2.10 allele was 72.1% in Tundra Nenets and 89.4% in Nganasans. The data obtained are consistent with the uniform geographic gradient of STin2 alleles, with low frequency of the STin2.10 allele in East Asia and its increase towards west.

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