Molecular markers based on SNPs in FAD3 genes for determination of linolenic acid content in flax seed

Poster (download)

399

Liubov Povkhova¹, Parfait Kezimana², Tatiana Rozhmina³, Elena Pushkova⁴, Roman Novakovskiy⁵, George Krasnov⁶, Alexey Dmitriev⁷, Nataliya Melnikova^8
1Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Russia, povhova.lv@phystech.edu
²Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; RUDN University, Moscow, Russia, k1par@mail.ru
³Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; Federal Research Center for Bast Fiber Crops, Torzhok, Russia, tatyana_rozhmina@mail.ru
⁴Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, pushkova18@gmail.com
⁵Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, 0legovich46@mail.ru
⁶Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, gskrasnov@mail.ru
⁷Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, alex_245@mail.ru
⁸Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, mnv-4529264@yandex.ru

Flax is grown for seeds in many countries. Depending on the ratio of fatty acids, seeds are used in pharmaceutical, food, feed, or polymer production. It is known that genes of FAD3 family responsible for the desaturation of linoleic (LIO) into linolenic (LIN) fatty acid and some alleles of these genes are associated with a certain composition of flax oil. However, the development of the marker-assisted selection of flax is complicated due to a lack of methods for the identification of these polymorphisms. In the present study, we used 84 flax cultivars/lines with diverse content of LIO and LIN to search for fatty acid associated SNPs and then developed HRM-based system for identification of these SNPs that can increase the efficiency of breeding by obtaining pure improved cultivars with the targeted LIO and LIN content.