Molecular markers based on SNPs in FAD3 genes for determination of linolenic acid content in flax seed

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Liubov Povkhova1, Parfait Kezimana2, Tatiana Rozhmina3, Elena Pushkova4, Roman Novakovskiy5, George Krasnov6, Alexey Dmitriev7, Nataliya Melnikova8
1Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Russia, povhova.lv@phystech.edu
2Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; RUDN University, Moscow, Russia, k1par@mail.ru
3Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia; Federal Research Center for Bast Fiber Crops, Torzhok, Russia, tatyana_rozhmina@mail.ru
4Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, pushkova18@gmail.com
5Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, 0legovich46@mail.ru
6Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, gskrasnov@mail.ru
7Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, alex_245@mail.ru
8Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, mnv-4529264@yandex.ru

Flax is grown for seeds in many countries. Depending on the ratio of fatty acids, seeds are used in pharmaceutical, food, feed, or polymer production. It is known that genes of FAD3 family responsible for the desaturation of linoleic (LIO) into linolenic (LIN) fatty acid and some alleles of these genes are associated with a certain composition of flax oil. However, the development of the marker-assisted selection of flax is complicated due to a lack of methods for the identification of these polymorphisms. In the present study, we used 84 flax cultivars/lines with diverse content of LIO and LIN to search for fatty acid associated SNPs and then developed HRM-based system for identification of these SNPs that can increase the efficiency of breeding by obtaining pure improved cultivars with the targeted LIO and LIN content.

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Lavrekha VV
Lavrekha VV
1 year ago

Did anybody use your test system for the identification of the three SNPs in FAD3 genes?

Liubov Povkhova
Liubov Povkhova
1 year ago
Reply to  Lavrekha VV

Dear Dr. Lavrekha,
Thank you for your question and sorry for the late response!
At present, the test-system is used in our joint research with the Institute for Flax (Torzhok, Russia). We are planning its wider application after the publication of the results.

Best regards,
Liubov