WebMCOT web-service for prediction of co-occurred DNA motifs in ChIP-seq data

Poster (download)

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Aleksey Mukhin¹, Victor Levitsky², Dmitriy Y. Oschepkov³, Sergey A. Lashin^4
1Institute Cytology and Genetics SB RAS Novosibirsk, Russia, mukhin@bionet.nsc.ru
²Institute Cytology and Genetics SB RAS, levitsky@bionet.nsc.ru
³Institute Cytology and Genetics SB RAS, diman@bionet.nsc.ru
⁴Institute Cytology and Genetics SB RAS, lashin@bionet.nsc.ru

Regulation of eukaryotic gene expression is controlled by specific regulatory proteins transcription factors. Binding sites of transcription factors are called motifs. Conventionally, genome-wide annotation of motifs performed with chromatin immunoprecipitation followed by massive sequencing (ChIP-seq) approach. The term composite element (CE) implied two closely located and frequently occurred in genomic DNA motifs. CEs contain two overlapping or spacing motifs. Earlier we proposed Motif Co-Occurrence Tool (MCOT) package that is capable of (a) predicting CEs with both overlapping and spacing of motifs in a single ChIP-seq dataset; (b) all predicted CEs were classified by conservation of both participant motifs. This work presents a web interface WebMCOT for MCOT package