Genetic mapping of QTLs controlling the ISIAH hypertensive rat behavior in an open field tests

Poster (download) Olga Redina1, Svetlana Smolenskaya2, Arcady Markel31Laboratory. of Evolutional Genetics, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia, oredina@bionet.nsc.ru2Laboratory of Evolutional Genetics, Wheat Genetics Laboratory, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia, svsmol@ngs.ru3Laboratory. of Evolutional Genetics, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia, markel@bionet.nsc.ru Genetic mapping of QTLs controlling the ISIAH hypertensive rat behavior in an open field tests evaluating research behavior, the level of anxiety and motor activity of animals was performed. The work was carried out using two groups of male hybrid rats F2(ISIAHxWAG) of different ages – 3-4 months (n = 103) and 6 months (n = 126) obtained by crossing hypertensive ISIAH and normotensive WAG rats whose behaviors are significantly different in all six tests in an open field taken in the analysis: the latency, the motor activity at 1-st minute of the test, motor activity on the periphery, grooming on the periphery, rearing on the periphery, defecation score. In both groups of rats, genetic loci associated with the studied traits of behavior were found and the common loci associated with both the behavioral traits and traits related to the hypertension development were identified. The results of our work suggest that genes that control behavioral patterns of ISIAH rats can either be closely linked to genes involved in the control of traits associated with the hypertensive status of these rats, or, in some loci, may have a pleiotropic effect on both behavioral traits and on the traits associated with the hypertensive status of ISIAH rats.

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lncRNAs – their potential in regulation of hypertension and behavior of ISIAH rats

Poster (download) Ivan Sidorenko1, Vladimir Babenko2, Arcady Markel3, Olga Redina41Institute of Cytology and Genetics SB RAS, vanyasidorenko22@gmail.com2Institute of Cytology and Genetics SB RAS, bob@bionet.nsc.ru3Institute of Cytology and Genetics SB RAS, markel@bionet.nsc.ru4Institute of Cytology and Genetics SB RAS, oredina@bionet.nsc.ru Long non-coding RNAs (lncRNAs) play an important role in the control of many biological processes in the body, including the development of cardiovascular diseases and hypertension. It is believed that lncRNAs play a central role in the epigenetic control of gene expression, however, the understanding of lncRNA biological functions and interactions is still far from being complete. In this work, we identified the lncRNAs differentially expressed in the hypothalami of hypertensive ISIAH and normotensive WAG rats, and revealed lncRNA-associated differentially expressed genes (DEGs) related to hypertension and behavioral characteristics of ISIAH rats (grooming, vertical activity, hyperactivity, abnormal emotion/affect behavior (including abnormal response to novelty). The work was carried out using transcriptome sequencing (RNA-Seq method). Three lncRNAs (Bc1, RGD1562890, and Snhg4) were found, the expression of which differed in the hypothalami of hypertensive ISIAH and normotensive WAG rats. The largest number of co-regulated genes, both associated with hypertension and behavior, was found for Snhg4. These findings may be useful for further understanding the role of lncRNAs in regulating the protein coding genes and modulating processes associated with both hypertension and behavior.

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