lncRNAs – their potential in regulation of hypertension and behavior of ISIAH rats

Poster (download)

194

Ivan Sidorenko¹, Vladimir Babenko², Arcady Markel³, Olga Redina^4
1Institute of Cytology and Genetics SB RAS, vanyasidorenko22@gmail.com
²Institute of Cytology and Genetics SB RAS, bob@bionet.nsc.ru
³Institute of Cytology and Genetics SB RAS, markel@bionet.nsc.ru
⁴Institute of Cytology and Genetics SB RAS, oredina@bionet.nsc.ru

Long non-coding RNAs (lncRNAs) play an important role in the control of many biological processes in the body, including the development of cardiovascular diseases and hypertension. It is believed that lncRNAs play a central role in the epigenetic control of gene expression, however, the understanding of lncRNA biological functions and interactions is still far from being complete. In this work, we identified the lncRNAs differentially expressed in the hypothalami of hypertensive ISIAH and normotensive WAG rats, and revealed lncRNA-associated differentially expressed genes (DEGs) related to hypertension and behavioral characteristics of ISIAH rats (grooming, vertical activity, hyperactivity, abnormal emotion/affect behavior (including abnormal response to novelty). The work was carried out using transcriptome sequencing (RNA-Seq method). Three lncRNAs (Bc1, RGD1562890, and Snhg4) were found, the expression of which differed in the hypothalami of hypertensive ISIAH and normotensive WAG rats. The largest number of co-regulated genes, both associated with hypertension and behavior, was found for Snhg4. These findings may be useful for further understanding the role of lncRNAs in regulating the protein coding genes and modulating processes associated with both hypertension and behavior.