lncRNAs – their potential in regulation of hypertension and behavior of ISIAH rats

Poster (download) Ivan Sidorenko1, Vladimir Babenko2, Arcady Markel3, Olga Redina41Institute of Cytology and Genetics SB RAS, vanyasidorenko22@gmail.com2Institute of Cytology and Genetics SB RAS, bob@bionet.nsc.ru3Institute of Cytology and Genetics SB RAS, markel@bionet.nsc.ru4Institute of Cytology and Genetics SB RAS, oredina@bionet.nsc.ru Long non-coding RNAs (lncRNAs) play an important role in the control of many biological processes in the body, including the development of cardiovascular diseases and hypertension. It is believed that lncRNAs play a central role in the epigenetic control of gene expression, however, the understanding of lncRNA biological functions and interactions is still far from being complete. In this work, we identified the lncRNAs differentially expressed in the hypothalami of hypertensive ISIAH and normotensive WAG rats, and revealed lncRNA-associated differentially expressed genes (DEGs) related to hypertension and behavioral characteristics of ISIAH rats (grooming, vertical activity, hyperactivity, abnormal emotion/affect behavior (including abnormal response to novelty). The work was carried out using transcriptome sequencing (RNA-Seq method). Three lncRNAs (Bc1, RGD1562890, and Snhg4) were found, the expression of which differed in the hypothalami of hypertensive ISIAH and normotensive WAG rats. The largest number of co-regulated genes, both associated with hypertension and behavior, was found for Snhg4. These findings may be useful for further understanding the role of lncRNAs in regulating the protein coding genes and modulating processes associated with both hypertension and behavior.

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Search for single nucleotide polymorphisms (SNPs) associated with hypertension in the genome of senescence-accelerated OXYS rats

Poster (download) Vasiliy A. Devyatkin1, Natalia A. Muraleva2, Olga E. Redina3, Nataliya Kolosova41Institute of Cytology and Genetics SB RAS, devyatkin@bionet.nsc.ru2Institute of Cytology and Genetics SB RAS, myraleva@bionet.nsc.ru3Institute of Cytology and Genetics SB RAS, oredina@bionet.nsc.ru4Institute of Cytology and Genetics SB RAS, Nnnnn80@ngs.ru Aging is a risk factor for many diseases, but the likelihood of developing with age also depends on genetic factors, environmental conditions, lifestyle, and the presence of other pathologies. The OXYS rat strain (ICG SB RAS) is a unique model for studying the mechanisms of aging, as already at an early age these animals develop a whole complex of age-dependent diseases, including cataracts, retinopathy, osteoporosis, hypertension and Alzheimer’s-like pathology. Although hypertension has risk factors typical of age-related diseases, it itself is a risk factor for many other pathologies. However, the complex senile phenotype does not appear in other hypertensive models, even with higher blood pressure. The aim of this study was, based on the results of RNA-Seq, the search for single nucleotide polymorphisms that could contribute to the development of hypertension in OXYS rats with accelerated aging. We found that OXYS rats are genetically far from other strains and presumably have their own bases for the development of hypertension, which may determine the absence of the senile phenotype of OXYS rats in hypertensive rat strains.

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