Poster (download) Elena Pushkova1, George Krasnov2, Roman Novakovskiy3, Nadezhda Bolsheva4, Nataliya Melnikova5, Alexey Dmitriev61Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, pushkova18@gmail.com2Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, gskrasnov@mail.ru3Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, 0legovich46@mail.ru4Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, nlbolsheva@mail.ru5Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, mnv-4529264@yandex.ru6Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, alex_245@mail.ru In the genomic analysis of trees, species belonging to the Populus genera serve as model objects. In our work, we carried out sequencing of the genome of male and female plants of Populus x sibirica, which are widespread in the Moscow region. Achieve high quality de novo In assemblies, we used a combination of two platforms, namely Oxford Nanopore and Illumina, to obtain long and high-precision short read operations, respectively. In general, using the MinION sequencer, we got 18 GB with an average N50 of 25 kb for each genotype, while using the HiSeq 2500 tool, we got from 36 to 52 million paired 125 + 125 readings for male and female plants. The Shasta assembler provided the best assembly results: N50 values were around 0.5 MB, and integrity was above 95% according to BUSCO. Analysis of the high-quality genomes of male and female P. x sibirica trees makes it possible to identify genes associated with poplar sex, which will facilitate the determination of its sex determination mechanism.
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