The characteristics of interaction of miRNA with mRNA of C2H2, ERF and GRAS transcription factors of arabidopsis, rice and maize

Rakhmetullina Aizhan1, Turasheva Svetlana2, Pyrkova Anna31SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, zhanullina1994@gmail.com2SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, svetlana.turasheva@kaznu.kz3SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, anna.pyrkova@kaznu.kz The miRNA binding sites with mRNA of genes encoding C2H2, ERF, GRAS transcription factors (TFs) were identified for Arabidopsis thaliana, Oryza sativa and Zea mays. The free energy (ΔG) of interaction of miRNA with mRNA target genes, the maximum of free energy (ΔGm), the ratio ΔG/ΔGm, and location of the potential binding sites were calculated using MirTarget program. In mRNA of C2H2, ERF, GRAS genes of all studied plants, miRNA binding sites were located in the protein-coding part (CDS) and 5’-untranslated region (5’UTR). The ath-miR5658-5p, ath-miR5021-5p, osa-miR2102-5p, osa-miR5075-3p had binding sites in mRNA of three studied families, with the value of ΔG/ΔGm from 91% to 98%. The miR171a-3p had binding sites in mRNA GRAS transcription factors family of all studied plants, with the value of ΔG/ΔGm equal 100%. The nucleotide sequences of ath-miR171a-3p, osa-miR171a-3p, and zma-miR171a-3p were similar, and their quantitative characteristics of interaction with mRNA of LOC_Os02g44360.1, GRMZM2G037792_P01, and AT2G45160.1 genes, were also similar. The obtained results indicate the dependence of expression TF of C2H2, ERF, GRAS families on miRNA.  

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Reconstruction and Analysis of Regulatory Gene Networks Involving Human Genes Associated with Main Forms of Pathozoospermia

Poster (download) Elena V. Ignatieva1, Alexander V. Osadchuk2, Maxim A Kleshev3, Ludmila V. Osadchuk41The Federal Research Center Institute of Cytology and Genetics, SB RAS, eignat@bionet.nsc.ru2The Federal Research Center Institute of Cytology and Genetics, SB RAS, osadchuk@bionet.nsc.ru3The Federal Research Center Institute of Cytology and Genetics, SB RAS, max82cll@bionet.nsc.ru4The Federal Research Center Institute of Cytology and Genetics, SB RAS, losadch@bionet.nsc.ru The study of the molecular-genetic mechanisms predisposing to decline in male reproductive potential (spermatogenic failure) is an actual problem of reproductive biology. Most often in laboratory studies evaluating male fertility, a study of the quality of ejaculate is used. Thus, a pathological condition called pathozoospermia can be detected if the quality indicators of the ejaculate are decreased. Pathozoospermia can manifest itself in several distinct forms, may occur in many diseases and can be caused by many factors, including genetic ones. To reveal regulatory interactions between genes associated with pathozoospermia, we reconstructed gene regulatory network involving genes harboring allelic variants associated with pathozoospermia. Regulatory network comprised seven genes encoding transcription factors (TFs) for which a set of target genes were predicted by MoLoTool web-service. We identified three key regulatory transcription factors (WT1, AHR, NR0B1) that have the greatest number of target genes in the network. Genes encoding these factors can be considered as the most promising candidate genes for identifying genetic variants associated with pathozoospermia.

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