The characteristics of interaction of miRNA with mRNA of C2H2, ERF and GRAS transcription factors of arabidopsis, rice and maize

Rakhmetullina Aizhan¹, Turasheva Svetlana², Pyrkova Anna^3
1SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, zhanullina1994@gmail.com
²SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, svetlana.turasheva@kaznu.kz
³SRI of biology and biotechnology problems Al-Farabi Kazakh National University Almaty, Kazakhstan, anna.pyrkova@kaznu.kz

The miRNA binding sites with mRNA of genes encoding C2H2, ERF, GRAS transcription factors (TFs) were identified for Arabidopsis thaliana, Oryza sativa and Zea mays. The free energy (ΔG) of interaction of miRNA with mRNA target genes, the maximum of free energy (ΔGm), the ratio ΔG/ΔGm, and location of the potential binding sites were calculated using MirTarget program. In mRNA of C2H2, ERF, GRAS genes of all studied plants, miRNA binding sites were located in the protein-coding part (CDS) and 5’-untranslated region (5’UTR). The ath-miR5658-5p, ath-miR5021-5p, osa-miR2102-5p, osa-miR5075-3p had binding sites in mRNA of three studied families, with the value of ΔG/ΔGm from 91% to 98%. The miR171a-3p had binding sites in mRNA GRAS transcription factors family of all studied plants, with the value of ΔG/ΔGm equal 100%. The nucleotide sequences of ath-miR171a-3p, osa-miR171a-3p, and zma-miR171a-3p were similar, and their quantitative characteristics of interaction with mRNA of LOC_Os02g44360.1, GRMZM2G037792_P01, and AT2G45160.1 genes, were also similar. The obtained results indicate the dependence of expression TF of C2H2, ERF, GRAS families on miRNA.