Poster (download)
16
Alexandr Poveshchenko1
1Research Institute of Clinical andExperimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russia, poveshchenkoa200@mail.ru
Breast cancer was induced by the administration of n-methyl-N-nitrosourea to Wistar rats. Some animals underwent only surgical intervention or only polychemotherapy (cyclophosphamide, methotrexate, 5-ftorouracil). In some animals, both types of therapy were combined, and in a separate group, the administration of the Panagen drug, which was a fragmented DNA molecule, was added to polychemotherapy. To study the concentration of cytokines in lymph, we used the Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad, USA). It was shown that in rats with breast cancer the content in the lymph and blood of most of the studied cytokines such as IL-1ОІ, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1О±, MIP-3О±, RANTES, TNF-О±, MCP-1 were statistically significantly higher, and IL-10 and GRO / KC, lower than in intact animals. Surgical removal of the tumor led to a significant decrease in the content of both pro-inflammatory and anti-inflammatory cytokines in the lymph. PCT led to a significant decrease in the content of IL-1ОІ, IL-4, IL-6, IL-7, MIP-1О±, MIP-3О±, RANTES in the blood serum and lymph of rats with breast cancer.
Tnank you veru much for sweethearted presentation. In the conclusion you claim that the cytokines IL-1β, IL-2, IL-12, IL-18, TNF-α, chemokines MIP-1α, MIP-3α, RANTES, IL-4, IL-13 are produced by cancer cells in breast cancer . At the same time, many of these cytokines increase when treated with Panagen after surgical removal of the tumor and PCT. This is reflects the activation of the immune system on your opininon. How do you explain this fact?
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