Molecular basis of phosphoryl guanidine oligonucleotides elongation by Taq DNA polymerase

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Alexander Lomzov^1
1ICBFM SB RAS, lomzov@niboch.nsc.ru

Phosphoryl guanidine oligonucleotides (PGO) become essential tools for basic research in molecular biology, molecular diagnostics and perspective for the therapeutics. To determine molecular basis of the observed effects of PG modification on elongation efficiency we performed molecular dynamics simulation of Taq polymerase / PGO complexes. The data obtained shows most important points for the analysis of protein / PGO interaction and gives useful information for development of improved PCR analysis.