Interplay between 5-HT and BDNF system in recombinant mouse strain upon chronic fluoxetine administration

Poster (download) Rodnyy A.Ya.1, Kondaurova E.M2, Antonov Y.V.3, Ilchibaeva T.V.4, Tsybko A.S.5, Naumenko V.S.61Institute of Cytology and Genetics Novosibirsk, Russia, aleksandr1994rodny@gmail.com2Institute of Cytology and Genetics Novosibirsk, Russia, kond_em@bionet.nsc.ru3Institute of Cytology and Genetics Novosibirsk, Russia, yegor@bionet.nsc.ru4Institute of Cytology and Genetics Novosibirsk, Russia, rbicehok@mail.ru5Institute of Cytology and Genetics Novosibirsk, Russia, antontsybko@bionet.nsc.ru6Institute of Cytology and Genetics Novosibirsk, Russia, naumenko2002@bionet.nsc.ru BDNF plays a key role in the development, differentiation, synaptogenesis and survival of brain neurons and in the processes of their adaptation to external impacts. Serotonergic (5-HT) system is another basic player in brain development and neuroplasticity. The study presents a comparative analysis of chronic fluoxetine treatment in recombinant mice differing in distal chromosome 13 fragment containing Htr1A gene of CBA mice strain on C57Bl6 genetic background. The problem here to be studied is mechanism of BDNF and 5-HT systems` interactions in antidepressant insensitivity. We measured mRNA and protein levels of BDNF, p75NTR, TrkB, 5-HT1A and 5-HT7 receptors, levels of 5-HT and its primary metabolite 5-Hydroxyindoleacetic acid (5-HIAA) in the brain structures that could have play primary role in mechanism of depression – frontal cortex and hippocampus. At the heart of the discussion are the different changes in BDNF system as well as in 5-HT system which allows us to conclude that the chronic fluoxetine injection increased depressive-like behavior in recombinant mice carrying distal chromosome 13 fragment containing Htr1A gene of CBA mice.

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РЎognitive functions and polymorphism of the BDNF gene in patients with schizophrenia and healthy individuals

Poster (download) Anastasiia Boiko1, Ekaterina Mikhalitskaya2, Elena Kornetova3, Svetlana Ivanova41Mental Health Research Institute Tomsk NRMC, anastasya-iv@yandex.ru2Mental Health Research Institute Tomsk NRMC, uzen63@mail.ru3Mental Health Research Institute Tomsk NRMC, kornetova@sibmail.com4Mental Health Research Institute Tomsk NRMC, ivanovaniipz@gmail.com In a number of schizophrenia concepts, the neurocognitive deficiency is distinguished to a separate domain along with positive and negative disorders and it is an important predictor of the unfavorable prognosis of the course of the disease and the development of persistent social dysfunction. The contribution of genetic mechanisms has been proven in the variability of attention instability and long-term memory impairment. Brain derived neurotrophic factor (BDNF) plays an important role in cell differentiation, survival, long-term potentiation, synaptic plasticity, learning and memory. The aim of the research is to study cognitive functions in patients with schizophrenia and healthy persons, and identify associations between cognitive function and polymorphism of BDNF gene. It was a complex clinical and biological examination of 550 patients with schizophrenia and 485 healthy persons. Significant differences in the distribution of genotypes of polymorphism rs6265 in patients with schizophrenia and healthy individuals were not found. Assessment of cognitive functions was carried out in 160 patients with schizophrenia and 106 healthy individuals using the BACS scale. The data obtained indicate that according to all BACS tests, the indicators of cognitive function in patients with schizophrenia are significantly worse than in healthy controls. Associations of genotypes of polymorphism rs6265 of the BDNF gene were detected by performing subtests of the BACS scale.

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