Results of the whole-genomic sequencing and annotation of the Listeria phenotype

Poster (download) Marina Terekhova11St. Petersburg State University of Information Technologies, Mechanics and Optics, Saint-Petersburg, Russia
, n.mim@mail.ru The incidence of Listeriosis in recent years is constantly increasing. So, in highly developed countries it ranges from 0.3 to 1.5, while in Russia – only 0.04%[1]. Moreover, the indicators in Moscow and St. Petersburg exceed those in the whole country by 4 times, which is explained by the different quality of clinical and laboratory diagnostics. Moreover, mortality due to complications of listeriosis infection can reach 30% [2]. Therefore, the fact of contamination of food products with listeria is of great concern. In this case, the strains of the species L. monocytogenes, L. innocua, L. ivanovii, L. grayii, L. seeligeri, L. welshimeriare most often distinguished. And if earlier it was believed that representatives of the species L. monocytogenespossess the largest set of pathogenicity factors, then the possibilities of molecular and bioinformatics research methods provide a reliable picture with respect to other representatives of the genus Listeria, in particular L. innocua.

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Differentially expressed genes associated with TMPRSS2-ERG molecular subtype of prostate cancer

Poster (download) Anastasiya Andreevna Kobelyatskaya1, Elena Anatolevna Pudova2, George Sergeevich Krasnov3, Anna Victorovna Kudryavtseva4, Kirill Mikhailovich Nyushko5, Boris Yakovlevich Alekseev61EIMB RAS, kaa.chel@mail.ru2EIMB RAS, pudova_elena@inbox.ru3EIMB RAS, gskrasnov@mail.ru4EIMB RAS, rhizamoeba@mail.ru5FSBI NMRRC, kirandja@yandex.ru6FSBI NMRRC, mnioi@mail.ru Prostate cancer (PC) is one of the most common and socially significant oncological diseases in men. This study examined the transcriptome profile of the most common molecular genetic subtype of prostate cancer, TMPRSS2-ERG. As a result of bioinformatics analysis conducted on the basis of The Cancer Genome Atlas project data, 115 differentially expressed genes were identified for this study group, and in particular, the most over-expressing genes were identified: ALOX15, CACNA1D, EML6, HLA-DMB, NKAIN1, OGDHL, PLA1A, SYT13. Enrichment pathways analysis showed that these genes are participants in important oncologically significant pathways, which emphasizes the association of this molecular subtype with an unfavorable prognosis for prostate cancer.

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Circulating microRNAs potentially associated with progression of castration-resistant prostate cancer

Elena Pudova11EIMB RAS, pudova_elena@inbox.ru Metastatic castration-resistant prostate cancer (mCRPC) is characterized by a high mortality rate due to its aggressiveness. In the field of modern oncourology, monitoring the activity of a disease for patients of this category during therapy is an important clinical problem, for the solution of which informative markers are needed. Circulating microRNAs can serve as such markers, which demonstrate diagnostic and prognostic potential in various types of cancer, including prostate cancer. The aim of this study is to search for circulating miRNAs of mCRPC patients plasma during therapy that are potentially associated with the progression of the disease, as well as to study the contribution of these miRNAs to the general progression mechanism. Based on high-throughput sequencing, a bioinformatic analysis was carried out with identification of the microRNA profile potentially associated with the progression of the disease (p-value <0.05): miR-221-3p, miR-99b-3p, miR-145-5p, miR-375-3p, miR-20a-5p, miR-21-5p, miR-451a, miR-199a-5p, miR-183-5p, miR-let-7i-5p and miR-let-7f-5p.

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Results of whole-genome sequencing and phenotype annotation of bacteria of the genus Listeria

Rogacheva Elizaveta11Saint-Petersburg Pasteur Institute, elizvla@yandex.ru The incidence of listeriosis in recent years is con- stantly increasing. So, in highly developed countries it ranges from 0.3 to 1.5%, while in Russia – only 0.04% [1]. Moreover, the indicators in Moscow and St. Petersburg exceed those in the whole country by 4 times, which is explained by the different quality of clinical and laboratory diagnostics. Moreover, mortality due to complications of listeriosis infection can reach 30% [2]. Therefore, the fact of contamination of food products with listeria is of great concern. In this case, the strains of the species L. monocytogenes, L. innocua, L. ivanovii, L. grayii, L. seeligeri, L. welshimeri are most often distinguished. And if earlier it was believed that representatives of the species L. monocytogenespossess the largest set of pathogenic factors, then the possibilities of molecular and bioinformatics research methods provide a reliable picture with respect to other representatives of the genusListeria, in particular L. innocua.

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