Bioinformatic Screening for Subtilisin-like Peptidases in Dikaryotic Fungi

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Nikita Alkin¹, Yakov Dunaevsky², Mikhail Belozersky³, Galina Beliakova⁴, Valeriia Tereshchenkova⁵, Elena Elpidina⁶
¹MSU Faculty of Biology, Moscow, Russia, nikita9801@mail.ru
²MSU Belozersky IPCB, Moscow, Russia, dun@belozersky.msu.ru
³MSU Belozersky IPCB, Moscow, Russia, mbeloz@belozersky.msu.ru
⁴MSU Faculty of Biology, Moscow, Russia, adm-odo@yandex.ru
⁵MSU Faculty of Chemistry, Moscow, Russia, v.tereshchenkova@gmail.com
⁶MSU Belozersky IPCB, Moscow, Russia, elp2@yandex.ru

Subtilisin-like peptidases (SLPs) play an important role in digestion and host colonization of pathogenic fungi. Analysis of 42 fungal genomes from all major taxa of Ascomycota and Basidiomycota was performed. Homologs of enzymes from all 4 major families of SLPs were found is the examined genomes. The most abundant types of the SLP homologs found were those of kexin and proteinase K families; pyrolysin homologs were detected in the half of the observed species; in addition, several homologs of OSP family proteins were found. Alignments of homologous amino acid sequences with type SLPs indicated presence of intact conserved domains and active sites. Number of SLP homologs differ distinctly between species from different environmental niches with the highest variety of SLPs found in pathogenic and parasitic fungi.