Semi-quantitative analysis of serum proteome in patients with bipolar disorder

Poster (download) Seregin Alexander Alexandrovich1, Smirnova Lyudmila Pavlovna2, Simutkin German Gennadievich31Mental Health Research Institute Tomsk National Research Medical Center of the Russian Academy of Sciences Tomsk, Russia, apocalips1991@mail.ru2Mental Health Research Institute Tomsk National Research Medical Center of the Russian Academy of Sciences Tomsk, Russia, lpsmirnova@yandex.ru3Mental Health Research Institute Tomsk National Research Medical Center of the Russian Academy of Sciences Tomsk, Russia, ggsimutkin@gmail.com The work analyzed the protein spectrum of blood serum of 45 with bipolar disorder. Protein recruitment in BD was mainly associated with the immune response, regulation of transport processes through the cell membrane and cellular communication, the development of neurons and oligodendrocytes, and cell growth. Cadherin-5 and vascular endothelial growth factor receptor 1 are a marker of endothelial dysfunction. The detection of these proteins suggests the presence of endothelial dysfunction in the pathogenesis of BD. The detection of myelin 1 transcription factor and the literature data on the effect of mutations in the MYT1L gene on the risk of major depressive disorder suggest the MYT1 protein as a potential BD biomarker. The results may help to discover new pathways associated BD.

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Amino acid and acylcarnitine levels relate with chronic schizophrenia

Poster (download) Irina Mednova1, Alexandr Chernonosov2, Marat Kasakin3, Elena Kornetova4, Arkadiy Semke5, Nikolay Bokhan6, Vladimir Koval7, Svetlana Ivanova81Mental Health Research Institute, TNRMC, Tomsk, Russia, irinka145@yandex.ru2Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia, alexander.chernonosov@niboch.nsc.ru3Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia, kassakinm@gmail.com4Mental Health Research Institute, TNRMC, Tomsk, Russia, kornetova@sibmail.com5Mental Health Research Institute, TNRMC, Tomsk, Russia, asemke@mail.ru6Mental Health Research Institute, TNRMC, Tomsk, Russia, mental@tnimc.ru7Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia, koval@niboch.nsc.ru8Mental Health Research Institute, TNRMC, Tomsk, Russia, ivanovaniipz@gmail.com Amino acids and acylcarnitines play important role as substrates and intermediate products in the most pathways related with schizophrenia. We found a significant decrease in the concentration of valine, aspartate, citrulline, glycine, arginine, glutamate and ornithine as well as C14, C14OH, C16OH, C16:1, C16:1OH, РЎ18, C18OH, C18:1, РЎ18:1OH, C18:2OH and C5:1 and increase C4DC in patients with schizophrenia in comparison to the controls.

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РЎognitive functions and polymorphism of the BDNF gene in patients with schizophrenia and healthy individuals

Poster (download) Anastasiia Boiko1, Ekaterina Mikhalitskaya2, Elena Kornetova3, Svetlana Ivanova41Mental Health Research Institute Tomsk NRMC, anastasya-iv@yandex.ru2Mental Health Research Institute Tomsk NRMC, uzen63@mail.ru3Mental Health Research Institute Tomsk NRMC, kornetova@sibmail.com4Mental Health Research Institute Tomsk NRMC, ivanovaniipz@gmail.com In a number of schizophrenia concepts, the neurocognitive deficiency is distinguished to a separate domain along with positive and negative disorders and it is an important predictor of the unfavorable prognosis of the course of the disease and the development of persistent social dysfunction. The contribution of genetic mechanisms has been proven in the variability of attention instability and long-term memory impairment. Brain derived neurotrophic factor (BDNF) plays an important role in cell differentiation, survival, long-term potentiation, synaptic plasticity, learning and memory. The aim of the research is to study cognitive functions in patients with schizophrenia and healthy persons, and identify associations between cognitive function and polymorphism of BDNF gene. It was a complex clinical and biological examination of 550 patients with schizophrenia and 485 healthy persons. Significant differences in the distribution of genotypes of polymorphism rs6265 in patients with schizophrenia and healthy individuals were not found. Assessment of cognitive functions was carried out in 160 patients with schizophrenia and 106 healthy individuals using the BACS scale. The data obtained indicate that according to all BACS tests, the indicators of cognitive function in patients with schizophrenia are significantly worse than in healthy controls. Associations of genotypes of polymorphism rs6265 of the BDNF gene were detected by performing subtests of the BACS scale.

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MBP-hydrolyzing abzymes as a peripheral markers associated with impaired myelination in schizophrenia

Poster (download) Video (download) Daria Parshukova1, Liudmila Smirnova2, Ekaterina Dmitrieva3, Arkady Semke4, Vasily Yarnykh5, Svetlana Ivanova61Mental Health Research Institute Tomsk National Research Medical Center RAS, Susl2008@yandex.ru2Mental Health Research Institute Tomsk National Research Medical Center RAS, Lpsmirnova@yandex.ru3Mental Health Research Institute Tomsk National Research Medical Center RAS, Egdtomsk@mail.ru4Mental Health Research Institute Tomsk National Research Medical Center RAS, Asemke@mail.ru5University of Washington, Department of Radiology, yarnykh@uw.edu6Mental Health Research Institute Tomsk National Research Medical Center RAS, Ivanovaniipz@gmail.com It is well-known that the pathology of myelin and oligodendrocytes is involved in the pathogenesis of schizophrenia. Anomalies in oligodendrocytes and myelin can be a source of neuronal disruption. The discovery of catalytic antibodies (abzymes) allows us to investigate their pathological role in various conditions. One of the possible ways of inducing proteolytic antibodies is the appearance in the peripheral blood of a substrate in the form of damaged protein fragments. In our previous study, it was shown that the IgG of schizophrenia patients can hydrolyze the myelin basic protein – one of the main components of the central nervous system myelin.В  The study of IgG proteases in accessible biomaterial (serum) and their association with myelination disturbance in schizophrenia may be potential criteria for monitoring the severity of mental disorders.

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Quantitative differences in the proteomic composition of the blood serum of patients with simple and paranoid schizophrenia

Poster (download) Video (download) Smirnova Liudmila, Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center, Tomsk, Russia, lpsmirnova@yandex.ru Semke Arkadiy, the Department of Endogenous Disorders Mental Health Research Institute of Tomsk National Research Medical Center. Tomsk, Russia, arkan1959@mail.ru Zgoda Victor, Laboratory of systems biology, Institute of Biomedical Chemistry, Moscow, Russia, vic@ibmh.msk.su Ivanova Svetlsna, Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center, Tomsk, Russia, ivanovaniipz@gmail.com Dmitrieva Elena, Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center, Tomsk, Russia, lena-312tom@yandex.ru The work analyzed the protein spectrum of blood serum of simple and paranoid schizophrenia. A set of proteins in schizophrenia was mainly associated with responsible for protein synthesis and the processes of transduction and translation; immune response, oxidative stress and apotosis, cell compataminization and cell contacts. In the blood serum of patients in the general group of schizophrenia, a significant increase in the number of RIPK1, mGluR6 receptors was revealed in comparison with healthy people. In addition, the amount of mGluR6 was significantly higher in patients with a simple form of schizophrenia than in patients with a paranoid form (p = 0.021). A quantitative assessment of the specific minor proteins that we studied using labeled standard peptides showed an increase in their number depending on the severity of the disease (a simple form of schizophrenia, leading negative symptoms)

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Immunoglobulins with proteolytic activity as a biomarker of impaired humoral immune system in schizophrenia

Poster (download) Video (download) Evgeny Ermakov1, Valentina Buneva2, Georgy Nevinsky31ICBFM SB RAS, Novosibirsk, Russia; NSU, Novosibirsk, Russia, evgeny_ermakov@mail.ru2ICBFM SB RAS, Novosibirsk, Russia; NSU, Novosibirsk, Russia, buneva@niboch.nsc.ru3ICBFM SB RAS, Novosibirsk, Russia; NSU, Novosibirsk, Russia, nevinsky@niboch.nsc.com Schizophrenia is known to be associated not only with imbalance in the neurotransmitter systems but also with immune system dysregulation. In this work, we found that pure polyclonal IgG preparations of patients with schizophrenia possess histone hydrolyzing proteolytic activity. The presence of proteolytic activity of IgGs is evidence of impaired humoral immune system in schizophrenia, which can be used as a biomarker for immunophenotyping of patients.

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