Comparative genomics of heat shock proteins system in extremophile nonbiting midges

Poster (download) Olga Kozlova1, Guzel Gazizova2, Elena Shagimardanova3, Oleg Gusev41Kazan Federal University, olga-sphinx@yandex.ru2Kazan Federal University, grgazizova@gmail.com3Kazan Federal University, rjuka@mail.ru4Kazan Federal University, gaijin.ru@gmail.com Here we compare the number and expression profiles of HSP-coding genes in larvae of Chironomidae family (Diptera), who are known for their ability to successfully combat abiotic stresses using wide range of behavioral, morphological and biochemical features. In order to perform comparative studies we sequenced and assembled genomes of 4 chironomids from different habitats and also sequenced whole-genome RNA of their larvae in control and stressed conditions. It was shown that compact genome sizes (up to 200 Mb) are typical for Chironomidae, while changes in size of a genome are mediated by elongation and shortening of introns length, as well as by changes in quantity and content of dispersed repeats. For all extremophile species under consideration species-specific gene expansion accompanied by formation of compact clusters in a genome was detected. The most amplitudinous reaction towards abiotic stress (desiccation) was shown by anhydrobiotic species Polypedilum vanderplanki (Africa). As for HSP-coding genes, it was noticed that genes of HSP20 and HSP70 show the most dramatic and universal up-regulation of expression in response to abiotic stress, while genes of chaperonins (HSP60) tend to be up-regulated in response to desiccation, but not to heat shock. But the most surprising notion was linked to acid-tolerant species Polipedilum cf. tamanigrum (Japan), because none of HSP-coding genes in this species showed statistically significant up-regulation, what may be explained by absence of special regulatory sequence – heat shock element (HSE) in their promotor regions.

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Genome-wide analysis of highly expressed plant retrotransposons

Murad Omarov1, Pavel Merkulov2, Sofia Gvaramia3, Liza Kolganova4, Ilya Kirov51All-Russia Research Institute of Agriculture biotechnology, muradok98@gmail.com2All-Russia Research Institute of Agriculture biotechnology, paulmerkulov97@gmail.com3All-Russia Research Institute of Agriculture biotechnology, sofia.gvaramia@gmail.com4All-Russia Research Institute of Agriculture biotechnology, liza.colg@gmail.com5All-Russia Research Institute of Agriculture biotechnology, kirovez@gmail.com Retrotransposons (TEs) are mobile genomic elements capable of transposition via reverse transcription of RNA intermediate. Transcription and mobility of TEs in a cell are under strong epigenetic silencing being partially recovered during stress and some development stages. But recent studies cast doubt on this axiomatic statement, revealing many transcripts of TEs (retrotranscriptome) in somatic organs under non-stressed conditions. The composition and structure of plant retrotranscriptome are still not clear. Here, we developed a pipeline for transcribed TEs identification and applied it to 7 plant species using RNA-seq data from different organs and under different conditions. Our results showed that TEs transcription under non-stressed conditions is the widespread phenomenon in plants and expressed TEs possess some distinctive genomic features

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Transcriptomic response of pea (Pisum sativum L.) plants to inoculation with nodule bacteria, arbuscular-mycorrhizal fungi and PGPB

Vladimir Zhukov1, Alexey Afonin2, Gulnar Akhtemova3, Andrei Bovin4, Aleksandra Dolgikh5, Artemii Gorshkov6, Emma Gribchenko7, Kira Ivanova8, Anna Kirienko9, Anna Kitaeva10, Marina Kliukova11, Olga Kulaeva12, Pyotr Kusakin13, Irina Leppyanen14, Olga Pavlova15, Daria Romanyuk16, Tatiana Serova17, Oksana Shtark18, Anton Sulima19, Anna Tsyganova20, Ekaterina Vasileva21, Aleksandr Zhernakov22, Evgeny Zorin23, Elena Dolgikh24, Viktor Tsyganov25, Igor Tikhonovich261ARRIAM, Saint-Petersburg, Russia, vzhukov@arriam.ru2ARRIAM, Saint-Petersburg, Russia, afoninalexeym@gmail.com3ARRIAM, Saint-Petersburg, Russia, ahgulya@yandex.ru4ARRIAM, Saint-Petersburg, Russia, andy-piter2007@mail.ru5ARRIAM, Saint-Petersburg, Russia, sqshadol@gmail.com6ARRIAM, Saint-Petersburg, Russia, artemius1993@yandex.ru7ARRIAM, Saint-Petersburg, Russia, gribemma@gmail.com8ARRIAM, Saint-Petersburg, Russia, kirakosmonavt_24@mail.ru9ARRIAM, Saint-Petersburg, Russia, kirienkoann@yandex.ru10ARRIAM, Saint-Petersburg, Russia, anykitaeva@gmail.com11ARRIAM, Saint-Petersburg, Russia, marina.kliukova@gmail.com12ARRIAM, Saint-Petersburg, Russia, okulaeva@arriam.ru13ARRIAM, Saint-Petersburg, Russia, kussakin@gmail.com14ARRIAM, Saint-Petersburg, Russia, leppyanen_irina@rambler.ru15ARRIAM, Saint-Petersburg, Russia, dobbi85@list.ru16ARRIAM, Saint-Petersburg, Russia, daria-rom@yandex.ru17ARRIAM, Saint-Petersburg, Russia, t_serova@rambler.ru18ARRIAM, Saint-Petersburg, Russia, oshtark@yandex.ru19ARRIAM, Saint-Petersburg, Russia, sulan555@mail.ru20ARRIAM, Saint-Petersburg, Russia, isaakij@mail.ru21ARRIAM, Saint-Petersburg, Russia, evasilieva@arriam.ru22ARRIAM, Saint-Petersburg, Russia, azhernakov@gmail.com23ARRIAM, Saint-Petersburg, Russia, kjokkjok8@gmail.com24ARRIAM, Saint-Petersburg, Russia, dol2helen@yahoo.com25ARRIAM, Saint-Petersburg, Russia, tsyganov@arriam.spb.ru26ARRIAM, Saint-Petersburg, Russia; Saint-Petersburg State University, Saint-Petersburg, Russia, arriam2008@yandex.ru Mutualistic symbioses formed by garden pea have been studied with use of transcriptomics in order to gain a new understanding of molecular mechanisms of beneficial effect that microsymbionts have on seed yield and quality.

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