How miRNAs can protect humans from coronaviruses COVID-19, SARS-CoV, and MERS-CoV

Anatoliy Ivashchenko1, Aizhan Rakhmetullina2, Aigul Akimniyazova3, Dana Aisina41Al-Farabi Kazakh National University, a.iavashchenko@gmail.com2Al-Farabi Kazakh National University, zhanullina1994@gmail.com3Al-Farabi Kazakh National University, akimniyazova@gmail.com4Al-Farabi Kazakh National University, dana.aisina03@gmail.com Viral diseases cause significant harm to human health and often cause high mortality. In the past twenty years, humanity has undergone infection by SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East respiratory syndrome) and COVID-19 coronaviruses, which spread from animals to humans and from person to person. These diseases have led to large economic losses. To fight coronaviruses and other viruses, it is proposed to use miRNAs, which regulate protein synthesis at the translational level. Out of 2565 miRNAs, miR-4778-3p, miR-6864-5p and miR-5197-3p were identified as the most effectively interacting with the gRNA of SARS-CoV, MERS-CoV and COVID-19, respectively. Based on the miR-4778-3p, miR-6864-5p and miR-5197-3p sequences, complete complementary miRNA (cc-miR) binding sites in the gRNA coronaviruses were created. Detected binding sites of these cc-miRs did not form intramolecular complexes in the 2D structure of the gRNA of SARS-CoV, MERS-CoV, and COVID-19 with a value of more than 85%. Therefore, cc-miRs will bind gRNA at these sites without competition. The cc-miRs for SARS-CoV, MERS-CoV, and COVID-19 did not have target genes among the 17508 human coding genes with a О”G/О”Gm of more than 85%, which implies the absence of side effects of cc-miRs on the translation of human mRNAs. cc-miRs can be used as therapeutic agents by incorporating them into exosomes or other vesicles and introducing them into the blood or lung by inhalation. The introduction of cc-miR into the blood will suppress the reproduction of the virus in the blood and in all organs into which it can enter.

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Accumulation of oxidative hepatobiliary lesions during experimental opisthorchiasis

Oxana Zaparina1, Anna Kovner2, Maria Pakharukova3, Viacheslav Mordvinov41ICG SB RAS, zp.oksana.93@gmail.com2ICG SB RAS, kovner@bionet.nsc.ru3ICG SB RAS, NSU, pakharukova@bionet.nsc.ru4ICG SB RAS, mordvin@bionet.nsc.ru Opisthorchiasis caused by the liver fluke Opisthorchis felineus in the hepatobiliary system of humans and fish-eating mammals is widespread on Russian territory. Pathogenesis of opisthorchiasis is accompanied by structural and functional disorders of the liver, including the formation of foci of chronic inflammation, cholestasis, cholecystitis and precancerous changes in the epithelium of the bile ducts. In the result of study, we showed time- dependent oxidative lesions accumulation and hepatoprotective effect of various antioxidants  

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Genetic association of the rs4680 COMT and rs1044396 CHRNA4 with internet addiction in Siberian adolescents

Marina Smolnikova1, Sergey Tereshchenko21Laboratory of cell-molecular physiology and pathology Scientific Research Institute of Medical Problems of the North FRC KSC SB RAS Krasnoyarsk, Russia, smarinv@yandex.ru2Clinical department somatic and mental health of children Scientific Research Institute of Medical Problems of the North FRC KSC SB RAS Krasnoyarsk, Russia, legise@mail.ru Abstract As the use of the Internet grows, the issue of the pathological and problematic Internet use (PIU) as well as online games has been relevant. Research related to a family history of temperament and mental disorders has rapidly been developing. Having the genetic component of developing Internet addiction was shown by twin studies using various populations, however, to date, the specific genes involved in the mechanisms of such heritability are not exactly identified. Materials: Genotyping of rs4680 COMT and rs1044396 CHRNA4 in 345 adolescents with different severity of internet addiction (tested with Chen Internet Addiction Scale) were analyzed. Results: The predominant allelic variant rs4680*G COMT in Khakass teenagers regardless of the severity of Internet addiction compared with Russian teenagers have been shown. It is important that some significant differences have also been observed between Russian PIU (45.5%) and Khakas PIU (63.8%) (OR 0.47, p = 0.024). We revealed that rs1044396 CC genotype prevalence progressively increase with IA severity: AIU group (n=179) – 19.0%, MIU group (n=82) – 32.9%, and PIU group (n=84) – 34.5%. The CC variant of rs1044396 CHRNA4 showed significantly higher anxiety scores as compared with carriers of T-allele. Our results correspond to similar results in the adult in another populations and are pilot in Russia; they are important for understanding the pathogenesis of IA at an early stage of addiction formation.

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The Siberian multimodal brain tumor image segmentation dataset (SBT)

Poster (download) Sergey Golushko1, Mikhail Amelin2, Bair Tuchinov3, Evgeniya Amelina4, Nikolay Tolstokulakov5, Evgeniy Pavlovskiy6, Vladimir Groza71Novosibirsk State University, s.k.golushko@gmail.com2FSBI \”Federal Neurosurgical Center\”, amelin81@gmail.com3Novosibirsk State University, bairts@gmail.com4Novosibirsk State University, amelina.evgenia@gmail.com5Novosibirsk State University, n.tolstokulakov@g.nsu.ru6Novosibirsk State University, pavlovskiy@post.nsu.ru7Median Technologies, vladimir.groza@gmail.com Automatic brain tumor segmentation from CT or MRI scans is one of the crucial problems among other directions and domains where daily clinical workflow requires to put a lot of efforts while studying patients with various pathologies.In this paper, we report the results of the research project \”Brain Tumor Segmentation\” organized in conjunction with the Federal Neurosurgical Center. Several state-of-the-art tumor segmentation algorithms were applied to a set of 100 MRI scans of meningioma, neurinoma and glioma patients – manually annotated by up to three raters – and to 100 comparable scans obtained using the automated tumor multi-region segmentation. Quantitative evaluations revealed a considerable agreement between the human raters in segmenting various tumor sub-regions (Dice scores in the range 85-90\\%). We found that different algorithms worked best for different sub-regions, but no single algorithm ranked in the top for all subregions simultaneously.

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DNA damage to nervous tissue due to lead intoxication combined with glucose loading

Poster (download) Yakimova Natalya Leonidovna1, Andreeva Elizaveta Sergeevna2, Buinova Ekaterina Vladimirovna31Estern-Siberian Institute of Medical and Ecological Research, Angarsk, Russia, ynl-77@list.ru2Estern-Siberian Institute of Medical and Ecological Research, Angarsk, Russia, liza.2995@mail.ru3Estern-Siberian Institute of Medical and Ecological Research, Angarsk, Russia, byuncat@mail.ru The influence of heavy metals on various organs and systems is known. Diabetes disorders are also well understood. The component contribution of glucose loading was manifested in an increase in genotoxic lesions of nerve cells caused by lead intoxication. DNA damage in the tail of nerve cell comets after exposure to lead and glucose loading increased to 52.86 (13.16–73.34) percent compared to 25.28 (12.24–54.79) percent of people poisoned by lead. In animals exposed to lead and glucose, brain cells with high DNA damage in the tail of comets dominated the brain, 62.77 (54.68-70.87) percent of the cells were apoptosis-positive compared to 48.42 (38, 37-58.47) percent rats treated with lead acetate without glucose load.

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Influence of the Factors of Maternal Milieu on Taste Preferences and Metabolic Parameters in Mouse Male and Female Offspring

Poster (download) Video (download) Denisova Elena1, Makarova Elena2, Savinkova M3 1Laboratory of Physiological Genetics Institute of Cytology and Genetics SB RAS Novosibirsk, RussiaLaboratory of Physiological Genetics Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, elena_nsib@list.ru 2Laboratory of Physiological Genetics Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, enmakarova@gmail.com 3Department of Physiology Novosibirsk State University Novosibirsk, Russia, m.savinkova@g.nsu.ru   Waiting is currently the leader among noncommunicable diseases. Calorie overload causes the development of obesity. It was shown that the prenatal and early postnatal state affects the susceptibility to obesity and can affect taste preferences [1], however, the mechanisms mediating the maternal effect on taste preferences in the offspring are unknown. The adipose tissue leptin hormone may be one of the factors mediating the maternal effect on the phenotype of the offspring. It has been shown that increasing the level of mites in the blood of pregnant women [2, 3] and programming the effects of leptins can be different in the offspring of different sexes. It is not known whether this is due to the positive programmatic effect of maternal leptin with a predisposition to its influence on taste preferences. These studies were supposed to affect pregnancy, muscle metabolism, the frequency of burns caused by diet and taste preferences in offspring of different sexes.

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  • June 29, 2020
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Correlation between Cytokines Lymph and Mesenteric Lymph Nodes on Breast Cancer in Rat

Poster (download) Kazakov Oleg1, Poveshchenko Alexander2, Orlov Nikolai3, Kabakov Alexey4, Lykov Alexander5, Strunkin Dmitry6, Konenkov Vladimir71Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, kazakoff_oleg@mail.ru2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, poveshchenkoa200@mail.ru3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, nbo@ngs.ru4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, kabakov_av85@mail.ru5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, aplykov2@mail.ru6Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Strunkind@mail.ru7Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, vikonenkov@gmail.com The correlation analysis of the morphometry of mesenteric lymph nodes and cytokines of the chest duct lymph in chemically induced breast cancer was carried out. The study showed that in breast cancer, the activity of the local immune response in the lymph nodes is aimed at antitumor protection. In breast cancer, the area of the paracortical zone remains at the level of the intact group, the area of lymphoid nodules with germinative centers and the area of medullary substance are increased, the number of macrophages in the thymus-dependent zone and zone responsible for humoral immunity is increased. In breast cancer positive correlation were revealed: in the germinative centers and medullary substance – mitotically dividing cells with cytokine IL-5, medium lymphocytes with chemokine MIP-1О±, in the germinative centers – immunoblasts with cytokine GRO/KC, in the paracortical zone – chemokine MCP-1 with macrophages, reticular cells with IL-6 and M-CSF, in medullary substance – small lymphocytes and mature cells plasma cells (the number of which is decrease) with chemokine Gro/KC, that can be caused by their migration from lymph nodes.В 

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  • June 29, 2020
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Relationship Cytokines Lymphs and Mesenteric Lymph Nodes Rats at Chemotherapy Breast Cancer

Poster (download) Kazakov Oleg1, Poveshchenko Alexander2, Orlov Nikolai3, Kabakov Alexey4, Lykov Alexander5, Strunkin Dmitry6, Konenkov Vladimir71Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, kazakoff_oleg@mail.ru2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, poveshchenkoa200@mail.ru3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, nbo@ngs.ru4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, kabakov_av85@mail.ru5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, aplykov2@mail.ru6Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, Strunkind@mail.ru7Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, vikonenkov@gmail.com At correlating the morphometry of mesenteric lymph nodes and the concentration of cytokines in the lymph of the thoracic duct in breast cancer induced by intramammary administration of N-methyl-n-nitrosourea, chemotherapy according to the CMF scheme, compared with breast cancer without treatment, positive relationships were found that may indicate on increase in the immunomodulatory and antitumor action of cytokines. The correlation of interferon IFNОі with small lymphocytes (the number which increased) and macrophages in germinative centers and mitotic dividing cells in medullary substance, correlation in germinative centers of immunoblasts with MIP-1О± and increase of number small lymphocytes in the thymus dependent zone of lymph nodes, correlation in medullary substance of interleukin IL-17 with mature plasma cells (the number of which is increased), correlation of interleukin IL-18 with mature plasma cells in the medullary sinuses.

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MICRORNAS (221, 429) ARE CORRELATION WITH LYMPHOCYTES OF AXILLARY LYMPH NODES IN EXPERIMENTAL BREAST CANCER

Poster (download) Aleksey Kabakov1, Alexandr Lykov2, Oleg Kazakov3, Alexandr Poveshchenko4, Olga Poveshchenko5, Dmitriy Strunkin6, Lyudmila Gulyaeva7, Andrey Letyagin8, Vladimir Konenkov91RICEL-Branch of IC&G SB RAS, kabakov_av@mail.ru2RICEL-Branch of IC&G SB RAS, aplykov2@mail.ru3RICEL-Branch of IC&G SB RAS, kazakoff_oleg@mail.ru4RICEL-Branch of IC&G SB RAS, poveshchenkoa200@mail.ru5RICEL-Branch of IC&G SB RAS, poveschenkoov@yandex.ru6RIMBB FRC FTM, strunkind@mail.ru7RIMBB FRC FTM, gulyaeva@soramn.ru8RICEL-Branch of IC&G SB RAS, letyaginay@bionet.nsc.ru9RICEL-Branch of IC&G SB RAS, vikonenkov@gmail.com The study of concentration miRNA (21, 221, 222, and 429) in serum, lymph of the thoracic duct and breast cancer tissue, as well as the cellular composition of axillary lymph node in Wistar female rats in chemically induced breast cancer. Concentration microRNA (-221, -429) in the tissues breast cancer and lymph are correlated with a decrease in number of lymphocytes in medullary cords of axillary lymph nodes.

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Loci and genes involved in chronic musculoskeletal pain identified via analysis of genetically independent pain phenotypes

Yakov Tsepilov1, Maxim B. Freidin2, Alexandra S. Shadrina3, Sodbo Z. Sharapov4, Elizaveta E. Elgaeva5, Jan van Zundert6, Lennart С. Karssen7, Pradeep Suri8, Frances M.K. Williams9, Yurii S. Aulchenko101Novosibirsk State University, drosophila.simulans@gmail.com2King’s College London, maxim.freydin@kcl.ac.uk3Novosibirsk State University, weiner.alexserg@gmail.com4Novosibirsk State University, sharapovsodbo@gmail.com5Novosibirsk State University, elizabeth.elgaeva@gmail.com6Maastricht University Medical Centre, jan.vanzundert@zol.be7PolyOmica, l.c.karssen@polyomica.com8VA Puget Sound Health Care System, pradeepsuri1@gmail.com9King’s College London, frances.williams@kcl.ac.uk10Novosibirsk State University, y.s.aulchenko@polyomica.com We have evaluated four genetically independent pain phenotypes of four common chronic musculoskeletal pains (GIPs). We assume that the first GIP represents a biopsychological component of chronic musculoskeletal pain, related to physiological and psychological aspects and possibly reflecting pain perception and processing.

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