Distribution of cell junction proteins in the descending colon of Muc2 mice

by Saydakova Snezhanna Sergeevna | Ogienko Anna Aleksandrovna | Boldyreva Lidia Valerievna |
Kozhevnikova Elena Nikolaevna | Scientific Research Institute of Neurosciences and Medicine,
Novosibirsk, Russia | Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, Russia | Scientific
Research Institute of Neurosciences and Medicine, Novosibirsk, Russia | Scientific Research Institute of
Neurosciences and Medicine, Novosibirsk, Russia

 

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6 Comments on “Distribution of cell junction proteins in the descending colon of Muc2 mice”

  1. Dear Snezana
    Thank you for the nice presentation.
    I wonder what bacteria are in the gut of your animals?
    I suppose it is not gnotobotic model

    1. Hello, Alexander! You’re right, these are not gnotobiotic animals. Our mice have specific pathogen free status. If you are interested in particular composition of Muc2-knockout mice microbiota, you can visit my collegue’s poster: https://bgrssb.icgbio.ru/2022/2022/06/30/investigation-of-influence-of-the-gut-microbial-composition-associated-with-colitis-on-mice-behavior/
      3rd slide of it contains the graph with average relative abundance of top 10 abundant genera in C57BL/6, Muc2+/+ and Muc2-/- mice.

  2. Thank you!
    I have a question:
    Why does the mutant line of mice have thickened intestinal walls?

    1. Thank you for your question! Muc2-knockout mice have chronic systemic inflammation in their colon due to exposure of “naked” epithelia to luminal antigens. It leads to crypts hyperplasia, so the gut looks thicker.

  3. Thanks a lot for the great poster. My questions:
    1. What does the term “leaky gut” mean? Is it due to greater intestinal permeability or does it contain holes?
    2. What do the abbreviations mean JAM-A, ZO-1 and IBD on the third slide?

    1. Hello, Dmitriy. Thanks for your questions!
      1. Leaky gut implies to high intestinal permeability when intercellular contacts are disrupted and epithelium is loose, allowing luminal pathogens to overpass intestinal barrier. It does not contain permanent holes.
      2. IBD is “inflammatory bowel disease”. I am sorry, I forgot to mention this abbreviation on the second slide. JAM-A and ZO-1 are proteins of tight junctions. JAM-A is “Junctional adhesion molecule A” and ZO-1 is “Zonula occludens-1”. JAM-A is transmembrane protein, forming dimers between two adjacent cells. ZO-1 allows JAM-A and claudins to anchor on actin cytoskeleton.

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